Reagents
Mice
- Msi2 mutant mice, B6;CB-Msi2Gt(pU-21T)2Imeg (Msi2-/-) established by gene-trap mutagenesis
- Msi1 reporter mice, REM1 (Msi1eYFP/+), , generated by conventional gene targeting, heterozygous for Msi1 allele
- Msi2 reporter mice, REM2 (Msi2eGFP/+), generated by conventional gene targeting, heterozygous for Msi2 allele
- Msi1f/f (Msi1flox/flox) generated by conventional gene targeting by inserting LoxP sites around Exons 1-4
- Msi1-/- (Msi1-Okano)
- Msi2-/- (Msi2-Okano)
- Msi2flox/flox
- Msi2CreER
Plasmids
- Dm_msi_T – Express His tagged Drosophila musashi RNA binding domain
- Dm_msi_RRM1 – Express His tagged Drosophila musashi RRM1
- pET-22HT-MSI1(7-192)R53E/R61E – R53E R61E double mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)G35D – G35D mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-103) – Express the RRM1 of mouse MSI1 in bacterial cells for protein purification
- pET-22HT-MSI1(7-192)R53E – R53E mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)G64A – G64A mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)H83F – H83F mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)R61E – R61E mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)Q30D – Q30D mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)R53E/R59E/R61E – R53E R59E R61E triple mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)H83L – H83L mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192)G35E – G35E mutant of the mouse MSI1 RBD
- pET-22HT-MSI1(7-192) – Express the RBD of mouse MSI1 in bacterial cells for protein purification
- pET-22HT-MSI2(8-193) – Express the RBD of human MSI2 in bacterial cells for protein purification
- pCDH-CMV-MSI1(FL) – express mouse MSI1 in mammalian cells
- Hs_MSI2_RBD – Express His tagged human MSI2 RNA binding domain
