Domain Structure and Function
A, a stereo view of superposition of the main chains of 15 final structures. N and C indicate Lys-21 and Ala-95, respectively, and loops 3 and 4 are labeled. B, a stereo view of the main chain of a restrained energy minimized mean structure of RBD1. α-Helices and β-strands are colored red and cyan, respectively. The side chains of Phe-23, Phe-63, and Phe-65 are also shown. C, a stereo view of the main chain of a restrained energy minimized mean structure of RBD2 from Ref. 25. N and C indicate Lys-110 and Ala-184, respectively. The side chains of Phe-112, Phe-152, and Phe-154 are also shown. (Miyanoiri et al. 2003)
Related Publications
Darai, N., Mahalapbutr, P., Wolschann, P., Lee, V. S., Wolfinger, M. T., & Rungrotmongkol, T. 2022. Theoretical studies on RNA recognition by Musashi 1 RNA-binding protein. Scientific reports, 12(1), 12137.
[ PubMed ]Wang, J., Lan, L., Wu, X., Xu, L., & Miao, Y. 2021. Mechanism of RNA recognition by a Musashi RNA-binding protein. Current research in structural biology, 4, 10–20.
[ PubMed ]Chen, T. C., & Huang, J. R. 2020. Musashi-1: An Example of How Polyalanine Tracts Contribute to Self-Association in the Intrinsically Disordered Regions of RNA-Binding Proteins. International journal of molecular sciences, 21(7), 2289.
[ PubMed ]Lan, L., Xing, M., Kashipathy, M., Douglas, J., Gao, P., Battaile, K., Hanzlik, R., Lovell, S., & Xu, L. 2020. Crystal and solution structures of human oncoprotein Musashi-2 N-terminal RNA recognition motif 1. Proteins, 88(4), 573–583.
[ PubMed ]Zearfoss NR, Deveau LM, Clingman CC, Schmidt E, Johnson ES, Massi F, Ryder SP. 2014. A conserved three-nucleotide core motif defines Musashi RNA binding specificity. J Biol Chem. 289(51):35530-41
[ PubMed ]Ohyama T, Nagata T, Tsuda K, Kobayashi N, Imai T, Okano H, Yamazaki T, Katahira M. 2012. Structure of Musashi1 in a complex with target RNA: the role of aromatic stacking interactions. Nucleic Acids Res. 40(7):3218–31
[ PubMed ]Kawahara H, Okada Y, Imai T, Iwanami A, Mischel PS, Okano H. 2011. Musashi1 cooperates in abnormal cell lineage protein 28 (Lin28)-mediated let-7 family microRNA biogenesis in early neural differentiation. J. Biol. Chem. 286(18):16121–30
[ PubMed ]Kawahara H, Imai T, Imataka H, Tsujimoto M, Matsumoto K, Okano H. 2008. Neural RNA-binding protein Musashi1 inhibits translation initiation by competing with eIF4G for PABP. J. Cell Biol. 181(4):639–53
[ PubMed ]Miyanoiri Y, Kobayashi H, Imai T, Watanabe M, Nagata T, Uesugi S, Okano H, Katahira M. 2003. Origin of higher affinity to RNA of the N-terminal RNA-binding domain than that of the C-terminal one of a mouse neural protein, Musashi1, as revealed by comparison of their structures, modes of interaction, surface electrostatic potentials, and backbone dynamics. J. Biol. Chem. 278(42):41309–15
[ PubMed ]Cuadrado A, García-Fernández LF, Imai T, Okano H, Muñoz A. 2002. Regulation of tau RNA maturation by thyroid hormone is mediated by the neural RNA-binding protein Musashi-1. Mol. Cell. Neurosci. 20(2):198–210
[ PubMed ]Sakakibara S, Nakamura Y, Satoh H, Okano H. 2001. RNA-binding protein Musashi2: developmentally regulated expression in neural precursor cells and subpopulations of neurons in mammalian CNS. J. Neurosci. 21(20):8091–107
[ PubMed ]Good P, Yoda A, Sakakibara S, Yamamoto A, Imai T, Sawa H, Ikeuchi T, Tsuji S, Satoh H, Okano H. 1998. The human Musashi homolog 1 (MSI1) gene encoding the homologue of Musashi/Nrp-1, a neural RNA-binding protein putatively expressed in CNS stem cells and neural progenitor cells. Genomics 52(3):382–84
[ PubMed ]Nakamura M, Okano H, Blendy JA, Montell C. 1994. Musashi, a neural RNA-binding protein required for Drosophila adult external sensory organ development. Neuron 13(1):67–81
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